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• Catherine Greenhalgh - PhD Candidate
EMAIL: cag [at] utm.utoronto.ca
I am working on nonlinear multimodal microscopic imaging. I have been investigating cardiomyocytes imaged with simultaneous second harmonic generation (SHG), third harmonic generation (THG), and multiphoton excitation fluorescence (MPF) microscopy. Our home built microscope allows simultaneous collection of two or three different nonlinear signals. When examining two volumes collected simultaneously, our structural crosscorrelation image analysis allows us to find structures common to both signals and those that are unique. We are able to observe different organelles within the same cell at the same time. Simultaneous visualization of organelles also provides the opportunity to follow dynamic interactions between them. SHG and THG microscopy do not deposit energy into the system and thus eliminates phototoxic effects common to fluorescence microscopy. As my PhD progresses, I will continue to work on the microscope development, including a combined near and far field multimodal system, and applications for the structural and dynamic studies of biological systems.
2001 B.Sc. (Hons), Trent University, Physics Department (2001)
2002 M.Sc., University of Toronto (2002)
Barzda, V., C. Greenhalgh, et al. "Visualization of mitochondria in cardiomyocytes by simultaneous harmonic generation and fluorescence microscopy." In Preparation.
Barzda, V., C. Greenhalgh, et al. (2004). "Second- and third- harmonic generation and multiphoton excitation fluorescence microscopy for simultaneous imaging of cardiomyocytes." Proceedings of SPIE: Commercial and Biomedical Applications of Ultrafast Lasers IV 5340
C.A. Greenhalgh, R.F. Moll, G.N. Pearson and A.J. Slavin. Test of the Ardell distribution function for two-dimensional adsorbate islands using thermal desorption spectroscopy. Phys. Rev. B61, 13969-13972 (2000).
A.J. Slavin, V. Arcas, C.A. Greenhalgh, E.R. Irvine and D.B. Marshall, Theoretical model for the thermal conductivity of a packed bed of solid spheroids in the presence of a static gas, with no adjustable parameters except at low pressure and temperature. Int. J. of Heat and Mass Transfer 45, 4151-4161 (2002).