Department of Chemical and Physical Sciences @ University of Toronto
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Multimodal microscopy helps to visualize different organelles inside cardiomyocyte

Heart muscle cells have been imaged simultaneously with three different contrast mechanisms: fluorescence, second harmonic and third harmonic generation. Different cellular organelles were revealed and co-localized in 3-dimensional image. The example of 3-dimensional image shows myofibrils of cardiomyocyte. The apparent granules aligned periodically in rows correspond to the anisotropic bands of sarcomeres. The multimodal microscopy proved to be a very informative non-invasive imaging tool for biological applications. It provides more information than currently used multi-photon laser scanning microscopes. The results had been recently published in Proceedings of SPIE Vol. 5340, p. 96-103.

Last Modified: Friday 17th June, 2011.